The Transient expression's methodology varies on the organism to transform; while plants can be transformed with a construct introduced into Agrobacterium tumefaciens via agroinfiltration or floral dip, most animal cells would require a viral vector, which are tools commonly used by biologists to deliver genetic material into cells.
Transient expression, more frequently referred to "transient gene expression", is the temporary expression of genes that are expressed for a short time after a nucleic acid, most frequently plasmid DNA encoding an expression cassette, has been introduced into eukaryotic cells. Methodology varies depending on the organism to transform. While plants can be transformed with a construct introduced into Agrobacterium tumefaciens via agroinfiltration or floral dip, most animal cells would require a viral vector. Viral vectors are tools commonly used by molecular biologists to deliver genetic material into cells. 
