Kammala jumla mai zuwa: Rubuce-rubucen farko da suka shafi ƙauyukan Cossack: "stanitsa", sun faro ne daga 1549. A cikin shekara ta 1552 Don Cossacks a karkashin umurnin Ataman Susar Fedorov ya shiga rundunar sojojin Ivan the Terrible a lokacin yakin basasa.
Siege na Kazan a shekara ta 1552. A ranar 2 ga Yuni, 1556, rukunin Cossack na Ataman Lyapun Filimonov, tare da Sojojin Muscovites waɗanda suka haɗa da strelets, sun ci nasara kuma sun haɗa da Khanate na Astrakhan.