Rubuta ci gaba don wannan sakin layi - Rubuce-rubucen farko da suka shafi ƙauyukan Cossack: "stanitsa", sun dawo zuwa 1549. A shekara ta 1552 Don Cossacks karkashin umurnin Ataman Susar Fedorov ya shiga rundunar Ivan the Terrible a lokacin Siege na Kazan
a shekara ta 1552. A ranar 2 ga Yuni, 1556, rukunin Cossack na Ataman Lyapun Filimonov, tare da Sojojin Muscovites waɗanda suka haɗa da strelets, sun ci nasara kuma sun haɗa da Khanate na Astrakhan.