Sam Holbrook waxa uu xukumada ka bilaabay horyaalka Appalachian League sanadii 1990, ka dibna waxa uu xukumay horyaalada Midwest, Carolina, Texas, Eastern iyo International ka hor inta uusan ku biirin horyaalka Major League June, 1996. Holbrook wuxuu ka shaqeeyay xilli ciyaareedkii 1996 ee Horyaalka Mareykanka, waxaana ku xigay seddex xilli ciyaareed oo ka tirsan Horyaalka Qaranka. Sannadkii 1999, wuxuu ka mid ahaa 22 garsoore oo is casilaadooda la aqbalay (tababbarkii gorgortanka ee midowga ee fashilmay), waxaana dib loo shaqaaleysiiyay 2002, garsoorida ciyaaraha labada horyaal ee tan iyo markaas. Holbrook wuxuu garsoore ka ahaa shan Division Series (2005, 2007, 2010, 2013, 2019), afar League Championship Series (2008, 2009, 2011, 2012), iyo saddex World Series (2010, 2016
, 2019). Wuxuu sidoo kale garsoore ka ahaa ciyaartii 2004 MLB All-Star Game. Holbrook waxaa loo magacaabay madaxa shaqaalaha bishii Febraayo 2017 MLB. Waxaa ku soo biiray kooxdiisa 2017 Greg Gibson, Jim Wolf iyo D.J. Reyburn.