Qeybtan waxaa lagu daahirin karaa iyadoo la adeegsanayo ultracentrifugation, taas oo qaybinaysa qaybaha kala duwan ee unugyada qaybaha ay ku jiraan borotiinka daciifka ah; lipids membrane iyo borotiinka; organelles unugyada, iyo nucleic acids. Precipitation by habka loo yaqaano salting out waxay xoojin kartaa borotiinka ka soo baxa lysate this. Noocyo kala duwan oo chromatography ah ayaa markaa loo isticmaalaa in lagu go'doomiyo borotiinka ama borotiinka xiisaha leh ee ku saleysan waxyaabaha ay ka kooban yihiin molikulka, miisaanka molikulka iyo isku xidhka. Heerka nadiifinta waxaa lagu kormeeri karaa iyadoo la adeegsanayo noocyo kala duwan oo borotiinka ah haddii la doonayo in lagu burburiyo xuubka unugyada iyo waxyaabaha ku jira gudaha oo la sii daayo xal loo yaqaan 'crude lysate'.
waa in laga nadiifiyaa unugyada kale