Ma nuclease anocheka ma nucleotide kubva kumagumo eDNA tambo anonzi exonucleases, nepo ma endonucleases achicheka mukati mematanho. Ma nuclease anonyanya kushandiswa mu molecular biology ndiwo marambidzo endonucleases, ayo anocheka DNA pane zvakateedzana. Semuenzaniso, iyo EcoRV enzyme inoratidzwa kuruboshwe inoziva iyo 6-base nhevedzano 5′-GATC-3′ uye inoita kucheka pamutsetse wakatwasuka. Muchisikirwo, aya ma enzyme anodzivirira mabhakitiriya kubva kuhutachiona hwephage nekudyara iyo phage DNA kana ichipinda mubhakitiriya sero, sechikamu chehurongwa hwekurambidza. Mune tekinoroji nhevedzano, aya ma nucleases-akasarudzika anoshandiswa muDNA mamorekuru uye zvigunwe zvemunwe cloning Mubvunzo: Ndeapi marudzi ema nucleases?
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