Rangarira ndima iri pasi apa uye ipapo pindura mubvunzo gare gare mumutauro mumwe chetewo sechikamu:  Kupfuura: Muongororo yeRT- PCR, RNA yeutachiona inobviswa muplasma yomurwere uye inochengetwa ne reverse transcriptase (RT) kuti ishandure RNA yeutachiona kuva cDNA. Polymerase chain reaction (PCR) maitiro anobva ashandiswa, achishandisa maprimer maviri anoshanda chete kugenome yeutachiona. Mushure mokunge PCR yapedzwa, zvinogadzirwa neDNA zvinobva zvabatanidzwa kuti zvive ma oligonucleotide chaiwo akasungirirwa kumadziro emudziyo, uye zvino zvinoonekwa nechinhu chinobatanidzwa ne enzyme. Kuwanda kweutachiona huri mumuenzaniso kunogona kuyerwa zvakarurama zvakakwana kuti zvionekwe kuchinja katatu.  Mubvunzo: Enzyme ipi inoshandiswa pakuongorora RT-PCR?
reverse transcriptase