Rangarira ndima iri pasi apa uye ipapo pindura mubvunzo gare gare mumutauro mumwe chetewo sechikamu:  Kupfuura: Kuita in vitro kuongorora, puroteni inofanira kucheneswa kubva kune zvimwe zvikamu zvemasero. Izvi zvinowanzotanga nokuora kwemasero, uko membrane yesero inoputswa uye zviri mukati mayo zvinoburitswa mumushonga unonzi crude lysate. Musanganiswa unoguma wagona kunatswa uchishandisa ultracentrifugation, iyo inoparadzanisa zvikamu zvakasiyana-siyana zvemasero muzvikamu zvine mapuroteni anogadziriswa; membrane lipids nemapuroteni; cellular organelles, uye nucleic acids. Kunaya nomutoo unozivikanwa sokubvisa munyu kunogona kuisa mapuroteni anobva mu lysate iyi. Mhando dzakasiyana-siyana dzechromatography dzinobva dzashandiswa kuti dziparadzanise puroteni kana kuti mapuroteni anodiwa zvichienderana nezvinhu zvakadai sehuremu hwemamolecule, net charge uye binding affinity. Mwero wekunatswa unogona kuongororwa uchishandisa mhando dzakasiyana-siyana dzegel electrophoresis kana uremu hweprotein hunodiwa uye isoelectric point zvichizivikanwa, ne spectroscopy kana protein iine zviratidzo zve spectroscopic, kana ne enzyme assays kana protein iine enzymatic activity. Mukuwedzera, mapuroteni anogona kuparadzaniswa maererano nemutoro wawo achishandisa electrofocusing. Nokuda kwemapuroteni omuzvarirwo, nhevedzano yematanho okuchenesa ingadikanwa kuwana puroteni yakachena zvakakwana nokuda kwezvinoshandiswa murabhoritari. Kuti zvive nyore kuita izvi, mashandiro anoita majini anowanzoshandiswa kuwedzera zvinhu zvemakemikari kumapuroteni zvinoita kuti zvive nyore kuachenesa pasina kuchinja zvaari kana kuti zvaari kuita. Pano, "chinyorwa" chinoumbwa neakasarudzika amino acid akateedzana, kazhinji akateedzana ehistidine masara ("His-tag"), inosungirirwa kune imwe yekupedzisira yeprotein. Somugumisiro, apo lysate inopfuudzwa pamusoro pekromatography column ine nickel, histidine residues ligate nickel uye inosungirirwa kumutsara apo zvikamu zvisingazivikanwi zve lysate zvinopfuura zvisina kudzivirirwa. Zvinhu zvakasiyana-siyana zvakagadzirwa kuti zvibatsire vatsvakurudzi kunatsa mapuroteni chaiwo kubva mumisanganiswa yakaoma.  Mubvunzo: Kuti muongororwe in vitro, chii chinofanira kuitika kupuroteni yacho?
inofanira kucheneswa kubva kune zvimwe zvikamu zvemasero