Mushonga uyu unowanzotanga ne cell lysis, umo membrane yesero inoputsika uye zviri mukati mayo zvinoburitswa mumushonga unozivikanwa se raw lysate. Musanganiswa unoguma unogona kucheneswa uchishandisa ultracentrifugation, iyo inopatsanura zvikamu zvakasiyana-siyana zvemasero muzvikamu zvine mapuroteni anogadziriswa; membrane lipids nemapuroteni; cellular organelles, uye nucleic acids. Precipitation nenzira inozivikanwa se salting out inogona kuisa mapuroteni kubva kune iyi lysate. Mhando dzakasiyana-siyana dze chromatography dzinobva dzashandiswa kupatsanura protein kana mapuroteni ekufarira zvichienderana nemamwe maitiro akadai semamolecular weight, net uye binding affinity.
inofanira kucheneswa kubva kune zvimwe zvikamu zvemasero