Rangarira ndima iri pasi apa uye ipapo pindura mubvunzo gare gare mumutauro mumwe chetewo sechikamu:  Kupfuura: Maantibody chaiwo anogadzirwa nokujowa antigen mumhuka inoyamwisa, yakadai sembe, mbeva, tsuro, mbudzi, gwai, kana kuti bhiza nokuda kwemwero mukuru wemaantibody. Ropa rakasarudzwa kubva kumhuka idzi rine polyclonal antibodies - maantibodies akawanda anosunga kuantigen imwe chete - mu serum, iyo zvino inogona kunzi antiserum. Antigens inoiswawo muhuku kuti pave nemapolyclonal antibodies muzai redehwe. Kuti vawane antibody inonyatsoenderana neepitope imwe chete yeantigen, maantibody-secreting lymphocyte anobviswa pamhuka yacho oitwa kuti asafa nokuasanganisa nemutsara wemasero ekenza. Masero akasanganiswa anonzi hybridomas, uye acharamba achikura uye achibudisa maantibody mumuti. Masero ehybridoma ari oga anoparadzaniswa nokuregererwa kuti agadzire masero anogadzira maantibody akafanana; maantibody aya anonzi monoclonal antibodies. Polyclonal uye monoclonal antibodies inowanzosvinwa uchishandisa Protein A/G kana antigen-affinity chromatography. Mukutsvakurudza, maantibodies anotsveneswa anoshandiswa mumashandisirwo akawanda. Maantibody ekushandisa pakutsvakurudza anogona kuwanikwa zvakananga kubva kune vanopa maantibody, kana kuti nokushandisa injini yekutsvaga yakasarudzika. Maantibody ekutsvakurudza anowanzoshandiswa kuziva uye kuwana mapuroteni ari mukati nemukati memasero. Antibodies anoshandiswa mukuyerera cytometry kusiyanisa mhando dzemasero nemapuroteni avanoratidza; mhando dzakasiyana dzemasero dzinoratidza musanganiswa wakasiyana wemapoka emamorekuru ekusiyanisa pamusoro pawo, uye dzinogadzira mapuroteni akasiyana e intracellular uye anobuda. Zvinoshandiswawo pakuita immunoprecipitation kuti zvisiyane nemapuroteni uye chero chinhu chakabatana nawo (co-immunoprecipitation) kubva kune mamwe mamorekuru ari mucell lysate, mukuongorora kweWestern blot kuti vaone mapuroteni akaparadzaniswa neelectrophoresis, uye mukuita immunohistochemistry kana immunofluorescence kuti vaongorore kutaura kwemapuroteni muzvikamu zvetishu kana kuti vawane mapuroteni mukati memasero vachibatsirwa nemaikorosikopu. Mapuroteni anogonawo kuonekwa uye kuyerwa nemaantibody, achishandisa ELISA neELISPOT...  Mubvunzo: Rudzii rwokudziridza runoitwa nemasero ehybridoma ari oga?
kudzikisira cloning