Tha an ath-bhualadh seo air a chur air dòigh leis an enzyme serine palmitoyl transferase agus is e seo an ceum a tha a 'cuingealachadh astar an t-slighe. Air an làimh eile, tha 3-keto-dihydrosphingosine air a lùghdachadh gu dihydrosphingosine, a tha an uairsin air a leantainn le acylation leis an enzyme (dihydro) ceramide synthase gus dihydroceramide a thoirt gu buil. Tha an ath-bhualadh mu dheireadh gus ceramide a thoirt gu buil air a lùghdachadh le dihydroceramide desaturase. Tha an ath-bhualadh de novo de ceramide a 'tachairt anns an endoplasmic reticulum. Tha ceramide air a ghiùlan gu an t-seirbheis le bhith a' trafaicleadh ceirmeatach ceirmeataid no am pròtain spingolipid. Aon uair's gu bheil an ceirmeat ceirmeatach a' dèanamh dihydroceramide, faodaidh e a bhith air a bhith air a bhith air a dhèanamh
(dihydro) ceramide synthase