zuwa ga sashi a ƙasa sannan kuma amsa tambayar daga baya a cikin wannan harshe kamar yadda sashi: Passage: Nucleases sune enzymes da ke yanke DNA strands ta hanyar catalyzing hydrolysis na phosphodiester bonds. Nucleases da ke hydrolyse nucleotides daga ƙarshen DNA strands ana kiransu exonucleases, yayin da endonucleases suka yanke a cikin zaren. nucleases da aka fi amfani da su a cikin ilimin kwayoyin halitta sune ƙuntatawa endonucleases, waɗanda ke yanke DNA a takamaiman jerin. Misali, enzyme na EcoRV da aka nuna a hagu yana gane jerin 6-base 5′-GATC-3′ kuma yana yankewa a layin kwance. A yanayi, waɗannan enzymes suna kare kwayoyin cuta daga kamuwa da cuta ta hanyar narkar da DNA ta phage lokacin da ta shiga cikin kwayar kwayar kwayar cuta, a matsayin wani ɓangare na tsarin ƙuntatawa. A cikin fasahar fasaha, ana amfani da waɗannan nucleases na musamman a cikin kwayar DNA da kwayar DNA Tambaya: Menene enzyme na EcoRV yake ganewa?
Tsarin 6-base 5′-GATATC-3