Rubuta ci gaba don wannan sakin layi - Tare da farkon Yaƙin Duniya na 1 a 1914, Burdenko ya sake ba da kansa don gaba kuma ya shiga ƙungiyar Red Cross na Arewa maso Yammacin Yamma, yana shiga cikin Ayyukan East Prussian da Yaƙin Kogin Vistula
Ka yi haƙuri. Baya ga ayyukan likitan filin, ya kuma kwashe sojoji da suka ji rauni a karkashin wutar abokan gaba, ya shirya rarrabuwa, taimako da wuraren sutura, gami da sassan musamman ga sojoji da ke fama da ciki, huhu da raunin kwanyar. A karo na farko a likitancin yaƙi Burdenko ya yi amfani da taimakon farko don raunin kwanyar. Daga 1915 zuwa 1917, ya kuma yi aiki a matsayin mai ba da shawara na likitan tiyata, da farko a Kovno da Vilna Governorates, sannan a Rundunar Soja ta 2 da kuma asibitocin Riga daban-daban. A watan Maris na shekarar 1917, an nada shi Babban Jami'in Kula da Lafiya na Yakin, amma ya bar mukamin a watan Mayu saboda rashin jituwa da Gwamnatin wucin gadi ta Rasha kuma ya koma gaba.