Wannan tsari yawanci yana farawa tare da cell lysis, a cikin abin da wani cell ta membrane da aka katse da ciki abinda ke ciki saki a cikin wani bayani da aka sani da raw lysate. The sakamakon cakuda za a iya tsarkake ta yin amfani da ultracentrifugation, wanda fractionates da daban-daban cellular aka gyara a cikin fractions dauke da narkewa sunadarai; membrane lipids da sunadarai; cellular organelles, da nucleic acid. Precipitation da wani Hanyar da aka sani da salting fitar iya mayar da hankali da sunadarai daga wannan lysate. daban-daban iri chromatography aka sa'an nan amfani da su ware sunadarai ko sunadarai na sha'awa bisa ga irin kwayoyin affinities, net nauyi da kuma dauri.
dole ne a tsarkake shi daga sauran abubuwan sel