Kammala jumla mai zuwa: Bayan gwagwarmayar neman 'yancin kai, sabon Gwamnan Buenos Aires mai ci gaba, Martín Rodríguez, ya sanya Pergamino a matsayin Alkalin Zaman Lafiya a 1822 kuma an buɗe makarantar farko a 1828. Ba da daɗewa ba garin ya zama gida ga ɗakunan yanka da yawa kuma bayan hauhawar 1829 na Gwamna Juan Manuel de Rosas (wanda bukatun danginsa ya ta'allaka ne da kiwon shanu) ƙawancen ƙawance ya haɓaka tsakanin gwamna da manyan iyalai na Pergamino, Acevedos da Anchorenas; Tsarin mulkin Rosas, duk da haka, ya haifar da rufe makarantar a 1838. Bayan Gwamna Rosas '
Bayan juyin mulki na 1852, mazauna yankin sun hada kai da Bartolomé Mitre, fitaccen mai ba da shawara a madadin lardin Buenos Aires don samun 'yancin kai. An zabi Mitre gwamna a 1860 kuma Shugaban Argentina a 1862. Shugaba Mitre na 1862 kafa cibiyar farko ta Agronomy ta Argentina ya taimaka wajen haifar da ci gaban sabon aikin tattalin arziki a yankin da ke kusa da Pergamino: aikin gona mai zurfi. Jirgin farko na hatsi na Argentine da ya isa ba tare da lalacewa ba a Burtaniya a 1875 ya fara haɓaka aikin gona wanda ga Pergamino, ya haifar da noman masara da yawa. Ci gaban garin ya kara hanzari bayan isowar Railway ta Tsakiya ta Argentine a 1882, a kan hanyar zuwa Rosario. Pergamino, wanda yawan jama'arsa ya wuce 10,000 kuma gida ne ga kamfanoni sama da 90 da aka yi wa rajista, an sanya shi a matsayin birni a ranar 23 ga Oktoba, 1895.