N2 + 16ATP + 8e- + 8H + -> 2NH3 + H2 + 16ADP + 16PiThe tsari ne a haɗe zuwa hydrolysis na 16 kwatankwacin ATP kuma shi ne tare da co-kafa daya kwatankwacin H2. Da yawa daga cikin hira na N2 cikin ammoniya faruwa a wani karfe tari da ake kira FeMoco, wani raguwa ga baƙin ƙarfe-molybdenum cofactor. The inji gudana ta hanyar jerin protonation da rage matakai inda FeMoco aiki site hydrogenates da N2 substrate. A free-rayuwa diarophs, da nitrogenase-halitta ammoniya ne assimilated cikin glutamate ta hanyar glutamine synthetase / glutamatase. Microbial kwayoyin halittar da ake bukata domin nitrogen gyare-gyare suna yadu rarraba a cikin mafi bambancin muhallin kamar yadda oxygen-daidaitawa enzymes wanzu a cikin kwayoyin halitta, inda ana rage yawan nitrogen-daidaitawa enzymes wanzu kawai ta hanyar oxygen-daidaitawa yanayi. Don wannan dalili, ana kiran sunadaran nitrogen-daitawa enzym
yanayin anaerobic