Gregurick was a professor of computational biology at the University of Maryland, Baltimore County where her research interests included dynamics of large macromolecules. Her areas of expertise are computational biology, high performance computing, neutron scattering and bioinformatics.
Gregurick was a program manager for the United States Department of Energy (DOE) where she developed the information and data sharing policy for the agency’s Genomics Science Program and oversaw the development and implementation of the DOE Systems Biology Knowledgebase, a framework to integrate data, models, and simulations together for a better understanding of energy and environmental processes. 
Gregurick joined the National Institute of General Medical Sciences (NIGMS) in 2013. She was the division director for NIGMS biomedical technology, bioinformatics and computational biology (BBCB). Her mission in BBCB was to advance research in computational biology, behavioral and data sciences, mathematical and biostatistical methods, and biomedical technologies in support of the NIGMS mission to increase understanding of life processes. 
Gregurick assisted in the development of the National Institutes of Health's (NIH) Office of Data Science Strategy (ODSS) which was established in 2018. Starting in November 2018, she served as a senior advisor to ODSS until she was appointed by Francis Collins on September 16, 2019, as NIH associate director for data science and director of the ODSS. Gregurick succeeded Philip Bourne. She received the 2020 Leadership in Biological Sciences Award from the .