Gastric organoids recapitulate at least partly the physiology of the stomach. Gastric organoids have been generated directly from pluripotent stem cells through the temporal manipulation of the FGF, WNT, BMP, retinoic acid and EGF signalling pathways in three-dimensional culture conditions. Gastric organoids have also been generated using LGR5 expressing stomach adult stem cells. Gastric organoids have been used as model for the study of cancer  along with human disease and development. For example, one study  investigated the underlying genetic alterations behind a patients metastatic tumor population, and identified that unlike the patients primary tumor, the metastasis had both alleles of the TGFBR2 gene mutated. To further assess the role of TGFBR2 in the metastasis, the investigators created organoids where TGFBR2 expression is knocked down, through which they were able to demonstrate that reduced TGFBR2 activity leads to invasion and metastasis of cancerous tumors both in vitro and in vivo.