Hi, I’m Kilian from Oculyze and now we’re
going to show you a step-by-step guide on
how to use our device so you can get started 
with your measurements
The mobile device must be set-up before you can use the system
Once this is set-up and the Oculyze app is downloaded, you are ready to begin
Now, I’ll be showing you how to use the
Oculyze Fermentation Wine
Let’s begin with dilution
Dilution
This is what you’ll need to make sure 
your yeast is well mixed
Water
Cylinder
Yeast sample
Pipette
Step 1: fill your measuring cylinder with
99 milliliters of water
Step 2: fill your pasteur pipette with yeast
to 1 milliliter
Step 3: empty the pasteur pipette into the
measuring cylinder
Step 4: run the solution in and out of the
pipette three times to make sure it is completely empty
Step 5: take the pasteur pipette and stir a few times 
Now it’s diluted
Staining
In order to determine the viability 
of the yeast, it must be stained
We recommend using methylene violet
Oculyze also supports methylene blue staining
Here is what you will need for staining
Diluted yeast sample 
Methylene violet 
Pipette
Step 1: take 0.5 milliliters of the diluted yeast sample 
and put it into the reaction tube
Step 2: then take 0.5 milliliters of the methylene violet
solution and put it into the same reaction tube
Step 3: Mix the sample by running the solution in and out of the pipette a few times
Loading the chamber
Now, let’s load the chamber
If you're only measuring concentration, 
use the diluted sample
If you're measuring concentration and viability, use the stained sample
Use the pasteur pipette to load a small sample
Pipette this into either one of the chamber openings
capillary forces will pull the sample
through the chamber
If you’re doing concentration, allow
1 minute for the sample to settle
If you’re doing concentration and viability,
allow for a total of 5 minutes for the stain
 to take effect and for the sample to settle
Measuring
Now that the chamber is ready let’s start
measuring
Firstly, let’s plug our compatible device
into the microscope
The microscope is powered by the mobile device
and the red light will illuminate when the two are successfully connected
Step 1: open the app and check the settings
Here, you can change the language, how many
images you capture, chamber height
your choice of staining dye, cell size slider, units of
sugar density, sharpness indicator, resolution
manual counting function and warnings options
Step 2: On the main menu, you can choose to
either
Start a New Fermentation
Continue an Ongoing Fermentation
Complete a Single Measurement or
Check your history
To begin, we’re going to complete a single
measurement
Step 3: Here you check your concentration
or your concentration and viability
Since we have stained our sample with methylene
violet, we will be checking our concentration
and viability
Step 4: Place the sample chamber under the microscope
Slide the sample chamber up to
the first marking, lightly screwing it into
place turning the focusing wheel counter clockwise
Step 5: Continue focusing until cells come
into focus, you will feel the wheel tighten
slightly as the pad comes into contact with the chamber
Be sure not to over tighten, as this can damage the chamber or the optics
Step 6: Take the image
The sharpness indicator on the bottom left 
helps you to take optimally focused images
aim for the highest number possible
This number will differ from image to image
Focused images should look like
this
Step 7: Click “keep” if you are happy
with the image
Step 8: you must conduct this step five times
Turn the focusing wheel clockwise to release the chamber before moving it to the next marking
Click “keep” if you are happy with the image, 
each time moving the position of the
chamber to the respective marking
Step 9: In the sample field – enter the
name of your sample
Date and time are automatically entered
If you followed this video, you can
enter 1 part sample and 99 parts water for the dilution
and 1 to 1 for the sample’s
mix ratio of coloring agent
If required, you can change 
your start date of fermentation
Include whatever you would like in the comment section
we recommend where the sample was
taken from, the type of yeast and the generation of the yeast
Finally, press the “Next Step”
button
Your results will then be sent to the cloud for analysis with the results being sent back to your device
This analysis and reporting 
will normally take less than a minute
Review of results
The results of the analysis are easy to follow
On the top left of the screen in the burgundy circle is the concentration of the sample in million cells per milliliter
In the tiny yellow circle is the percentage of these cells that are budding
In the violet circle is the viability percentage
You can view the breakdown of cells by size 
with the histogram showing a distribution 
of the cell sizes in the sample
The burgundy bars indicate live cells, and the violet bars stacked on top show the distribution of dead cells
Scroll down further and you can see how the
algorithm has identified the cells
The green cells are the living cells, the violet cells
are dead, while the yellow colour indicates budding cells
The Pitch Rate Calculator 
is accessible through the gray arrow
New Fermentation
To track a fermentation from beginning to
end, you will to press “start a new fermentation”
Step 1: On this page, fill in as much information as you can to maintain accurate records
When completed, click the “Done” button
Your batch is now ready to be monitored
Step 2: To begin monitoring, click on “Ongoing Fermentation”
Then find the analysis you would like to continue tracking
Press the “plus” button
From here, it is like completing a single measurement
Complete the analysis like you normally would
You can continue adding analyses to your batch of fermenting wine until it has finished fermenting
You can check on the progress by click on
the fermenting batch name
Here you can check on the progress of your concentration, your viability and sugar density
By double clicking on a particular analysis, you can revisit the results
Step 3: Once the fermentation has finished,
press the “flag” button
and your fermentation will be finalized and sent to the history
History
The history enables the winemaker to check on past results of
Fermentations
Single Measurements and
Unfinished Analyses
For fermentations, click on the batch you
would like to see
There you can see all the details of what went into the batch along with a graph
which plots your concentration viability and sugar density from start to the finish
Simple double click on an analysis
to see what the individual result was
For single measurements, click on the analysis you want to check on
Finally, unfinished analyses will be listed
Maintenance
In order to guarantee optimal analysis, the
chamber must be cleaned and dried immediately
after each use
Step 1: Fill the syringe with distilled water
Step 2: Carefully rinse the chamber
Step 3: Use the bellow to dry the chamber
Carefully blow air through the chamber
and optionally use a small piece of tissue
or a cotton swab at the end to collect excessive water
Thanks for watching! Contact sales@oculyze.de for more information
