While the possible involvement of beta[unreadable]2[unreadable]-microglobulin in cytotoxic T lymphocytes (CTL) recognition events is still under investigation, with no conclusive results emerging from this study yet, concrete progress made in several other areas includes: (1) the dml mutant is shown to express a hybrid H-2D[unreadable]d[unreadable]/H-2L[unreadable]d[unreadable] protein that is probably the product of fused genes; (2) CTL responses to cell surface-coupled proteins can now be studied since we developed a way of covalently conjugating a million or more protein molecules to cells that then remain viable and stable for over a day in culture and that serve as excellent targets for lysis by antibody plus complement and ADCC; (3) we discovered that some monoclonal antibodies are excellent ADCC mediators and that ADCC efficiency depends both on the subclass (IgG2a or IgG3) and the orientation of the antibody on the cell; (4) normal human lymphocytes are not good ADCC targets; (5) human K effectors induce rapid fragmentation of the nuclear DNA in mouse target cells; and (6) nuclear DNA fragmentation is induced in mouse target cells only and not in human target cells when such targets are lysed by ADCC, CTL, or NK cells. (CS)
NIH ExPorter
NIH ExPorter